A tour of the Microbiology Lab

Isolation of bacterial colonies

Microbiology, which deals with diseases caused by infectious agents such as bacteria, viruses, fungi and parasites.

Medicine is Pathology
rcpa.edu.au

Go inside a clinical microbiology lab at Penn State Milton S. Hershey Medical Center and learn about careers in clinical microbiology.

A tour of a diagnostic medical microbiology highlighting some of the work done. With thanks to Susan UCLH/RBH.

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This video demonstrates basic microbiological aseptic techniques used for transferring bacteria from one culture to another. Techniques include use of a Bunsen burner, transferring bacterial cultures from Petri plate to a liquid culture or from liquid to liquid culture using inoculation loops or needles.

Recorded at LabPlus at Auckland hospital in New Zealand the video presents MLS as a career option in the health industry in New Zealand

All of the videos (in the microbiology lab playlist) prior to this one cover all of the labs for your first lab practical.

Tutorial on plate streaking; provides streaking instructions and the sterile techniques needed to successfully streak a plate of bacteria.

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Bowling Green State University Medical Laboratory Science students submission for the 2013 ASCLS viral video contest.

Song: Evgeny Grinko - Winter Sunshine. Licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 2.0 Germany License.

Tour of SCC microbiology lab, overview of safety and facility

Biomedical Science graduate Lauren Upston is passionate about her job as a Medical Scientist.

In the hospital we are working alongside everyone else to bring about a result for the patient - that’s really satisfying. Exciting things happen all the time. I’m still learning things every day.

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AKA Kirby-Bauer test. General Microbiology lab video from Nicolet College.

Order now at You can smell a good science project a block away. In this case, multiply the fun by six and you have one of our most popular science fair project kits. You'll get six large Petri dishes, 10 cm (4 in) in diameter, a microwave safe plastic beaker, cotton swabs and the highest quality nutrient agar. This is the same nutrient agar used by microbiologists to grow really funky stuff in the lab. The instructions give you great suggestions for collecting samples and ways to gross out your parents.

B.Sc MICROBIOLOGY CAREERS. Go through the career opportunities of B.Sc MICROBIOLOGY, Govt Jobs and Employment News channel from Freshersworld.com – The No.1 job portal for freshers in India. Visit for detailed Career information, Job Opportunities, Career growth, Salary Package ,Higher education details of B.Sc MICROBIOLOGY.
Just like how one professes and involves deeply in any niche of science, there is Microbiology. In this three year undergraduate course, you learn everything about Micro organisms and their importance in your life. It is detailed niche field of Science and students get to learn a lot about another variety of science.
A BSc in Microbiology is a detailed field of science where one gets to learn everything about the life and use of micro organisms. It is a very micro leveled study of science.
A student who has completed his study in Microbiology will have enormous openings in the Public sector firms and also get jobs in Private sector organizations. Various undertakings have opened up abroad that offer employment opportunities for Bachelor of Science in Microbiology graduates.
Private sector also provides a lot of job chances for Bachelor of Science in Microbiology pass outs. However, the most common field of work for Microbiology aspirants in this sector will be research organizations. They can also opt for taking up teaching profession as well. The salary packages of students doing BSc in Microbiology will depend on the job profile and also the area in which these graduates are working. They will not only work in the fields of Microbiology but also in some other allied fields if possible. Thus, you must look for that perfect opening and root for that:-
The most common job profiles for students of BSc in Microbiology in the Private sector units include:
• Medical Microbiologists
• Agricultural Microbiologists
• Industrial Microbiologists
• Marine Microbiologists
Apart from taking Bank Jobs or civil service exams, BSc in Microbiology will offer various options in proper Government jobs also. However, these graduates need to attend several tests that are conducted by Government boards to get well placed in these Public sector firms. Research fields however is a good option for these graduates. You will be mostly called a Microbiologist if having passed from this field. You can also make your job in teaching with Microbiology. Even that is yet another lucrative choice.
The salary packages are not so good at the start but it will gradually increase. A beginner in this field can earn in the range of Rs.10000/- to Rs.15000/- per month. With good experience and higher studies, one can attempt and even look forward to a higher remuneration as well.
The common areas of employment for students of BSc in Microbiology at the Government sector include:
• Development laboratories
• Food Industry
• Beverage Units
• Chemical Industries
• Hospitals
• Pharmaceutical Sector

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GHP provides Microbiological, Chemical, Physical, Environmental and Calibration testing through a state of art laboratory. The microbiological, chemical, physical, environmental and calibration laboratory offer a wide range of test and expertise. To learn more please contact GHP Food Testing & Calibration Lab.

* Microbiology
* Water Microbiology
* Individual Analyses
* Shelf-life Studies
* Chemical Analysis
* Nutritional Analysis & Labeling

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The most common method of separating bacteria cell on agar surface to obtain isolated colonies is the streak method to inoculate Petri dish. It provides a simple and a raid method of diluting the sample by mechanical means. As the loop is stretched across the agar surface more and more bacteria are separated until the required bacteria are deposited on the agar. After inoculation, the area at the beginning of the streak pattern with show confluent growth while the area at the end of the pattern should show the discreet colonies.

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The Gram staining method is named after the Danish bacteriologist Hans Christian Gram (1853 --1938) who originally devised it in 1882 (but published in 1884), to discriminate between pneumococci and Klebsiella pneumoniae bacteria in lung tissue. It is a differential staining method of differentiating bacterial species into two large groups (Gram-positive and Gram-negative) based on the chemical and physical properties of their cell walls. This reaction divides the eubacteria into
two fundamental groups according to their stainability and is one of the basic foundations on which bacterial identification is built. Gram staining is not used to classify archaea, since these microorganisms give very variable responses.

Part 1 of the lab review in microbiology

Part 2 here:
Part 3 here:

Experiments read and reviewed:
1.Motility Tall
2.Catalase
3.Reduction of nitrate
4.Hemolysis
-alpha,
-beta,
-gamma
5.CHO Fermentation
-mannitol,
-glucose,
-lactose,
-sucrose
-phenol red pH indicator
6.Unique source of Nitrogen
-ammonium phosphate (NH4)
-Brom cresol purple pH indicator

Disclaimer: For informational purpose. Please consult your lab notes and textbook for confirmation and for more detailed and complete information.

If you catch an uncorrected mistake please make a note below with correction, your sources, and the point in timeline (min:sec) where correction is needed. Thank you.

Part 2 will be edited and uploaded tomorrow.

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Microbiology, which deals with diseases caused by infectious agents such as bacteria, viruses, fungi and parasites.

Medicine is Pathology
rcpa.edu.au

Video describing the different parts of a compound microscope.

How to make liquid agar and use the autoclave

For information, there are many types of agar that use for culture bacteria..
In this video, we only share 5 types only..

1) TCBS Agar
2) TSA Agar
3) EMB Agar
4) MHA Agar
5) Lowenstein-Jensen Medium Agar

Enjoy k ^^

Links to the books shown in this video on Amazon:

Clinical Microbiology Made Ridiculously Simple


BRS Microbiology and Immunology


Review of Medical Microbiology and Immunology, Levinson


Links to products:

1) Step Up to Medicine



2) First Aid for the Wards



3) Kaplan USMLE Step 2 CK Lecture Notes (PDFs I Mentioned)



4) Maxwell Quick Medical Reference



5) Pocket Medicine (Either edition is fine)





6) Reflex Hammers









7) Tuning Forks







8) Pen Lights





9) Cheap Pens







10) Stethoscope









(Name tag)

11) Ophthalmoscopes



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How to Study for USMLE Step 1

1) First Aid for the USMLE Step 1

(2015)

(2016)

2) Pathoma - Fundamentals of Pathology


3) First Aid Q&A; for the USMLE Step 1


4) First Aid Cases for the USMLE Step 1


5) Kaplan USMLE Step 1 Qbook


6) USMLE Step 1 Secrets


7) USMLE Step 1 (Deja Review)


8) First Aid Basic Sciences


9) Repeated USMLE Questions


10) Crush Step 1


11) First Aid for the Wards


12) USMLE Step 2 CK Lecture Notes (PDFs I Mentioned)


13) USMLE Step 1 Made Ridiculously Simple


********

How to Study for USMLE Step 2 CK

Links to products mentioned in video:

1) Step-Up to USMLE Step 2 CK


2) Master the Boards USMLE Step 2 CK


3) First Aid for the USMLE Step 2 CK


4) USMLE Step 2 Secrets


5) Kaplan USMLE Step 2 CK Lecture Notes (PDFs I Mentioned)


6) Kaplan USMLE Step 2 CK QBook


7) Brochert's Crush Step 2


8) First Aid Cases for the USMLE Step 2 CK


9) Deja Review USMLE Step 2 CK


10) First Aid Q&A; for the USMLE Step 2 CK


11) Step-Up to Medicine


12) USMLE Step 2 Made Ridiculously Simple


*********

How to Study for USMLE Step 2 CS

Links to products mentioned in video:

1) First Aid for the USMLE Step 2 CS


2) Kaplan USMLE Step 2 CS Core Cases


3) Kaplan USMLE Step 2 CS Strategies


4) Kaplan USMLE Step 2 CS: Complex Cases


5) CS Checklists


6) Beat the USMLE STEP 2 CS


Keep in touch!

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The hemocytometer was invented by Louis-Charles Malassez. The device is basically a special microscope slide consists of two chambers, each of which is divided into nine 1.0mm x 1.0mm large squares separated from one another by triple lines. The area of each is 1mm2. A cover glass is supported over the chambers at a height of 0.1mm. Thus the entire counting grid lies under a volume of 0.9 mm3 on one side. Cell suspensions are introduced under the cover glass. The hemocytometer is placed on the microscope stage and the cell suspension is counted.

Introduction and uses of culture media for growing pathogenic bacteria and fungi

Medical laboratory technologists perform a variety of laboratory tests and procedures to assist physicians in diagnosing, monitoring, treating and preventing disease. For more information on this occupation see alis.alberta.ca/occinfo.

This is the introduction on general microbiology practical discussing culture media, culture techniques and explaining overview of microbiology lab.
For more information, log on to-

Download the study materials here-

Question source - indiabix.com

Microbiology lab tutorial on Mannitol Salt Agar (MSA) specialized bacterial growth medium. Examples of sterile MSA as well as plates with growth of halophilic mannitol fermenting bacteria (pathogen Staphylococcus aureus) and halophilic non-mannitol fermenting bacteria (normal flora S. epidermidis).

Video by Tami (Guy) Port, Chief Executive Nerd at ScienceProfOnline.com. For the lab materials that go with this movie, see the Virtual Microbiology Classroom Bacterial Growth Media and Culture Laboratory Main Page ( at ScienceProfOnline.com.

** OOPS! This is the re-release of the MSA video, previous version contained a typo - descendants was spelled incorrectly. **

( ) - Cells are cultured under précised conditions; A complete protocol on how to thaw, maintain and freeze attached cells is shown in this AbVideo. More videos at Abnova

The Department of Bioengineering at U of Illinois has provided an awesome facility for students to learn the art and science of cell culture and tissue engineering. Joanne takes you on a tour. Videographer is my good friend, visit his site, his photography is awesome.

This video describes the importance of using wastewater microbes as indicator organisms to help optimize your wastewater treatment system.

Master list of Dead Scientists and microbiologists


ABC News
September 22, 2009

September 21, 2009 (CHICAGO) (WLS) -- A University of Chicago researcher died after being exposed to a strain of bacteria that causes plague. And Malcolm Casadaban's family wants answers.

The Chicago Department of Public Health says there's no sign anyone else was exposed to the bacteria.

The tragic irony is that Professor Casadaban had been trying to develop a vaccine so that thousands of people around the world wouldn't die a painful, ugly death from a bacterial infection related to the plague. But it was that bacterium that appears to have killed him

We really thought he was so strong, we didn't see this coming at all, said Leigh Casadaban, daughter.

Not only did they not see it coming, but sisters Leigh and Brooke Casadaban say their father never knew what killed him.

It was just so much of a shock, even the time he was in the ER, from the moment he got accepted to the time he passed away. We had no idea, we didn't even get to say goodbye to him, said Brooke Casadaban.
Malcom Casadaban was a professor of molecular genetics at the University of Chicago for 30 years.

For the past eight years, his daughters say, he'd been working with a strain of bacteria called yersinia pestis, trying to develop a stronger vaccine for the plague, once the world's worst health scourge.

The weakened strain he was using, however, isn't supposed to make healthy people sick.

And that is the big mystery now for investigators with the Centers for Disease Control and the state and city public health departments who've been looking for clues at the U. of C. labs.

Was it a change in the organism, something about the person infected that made him uniquely susceptible? We don't really know the answers yet, said John Easton, University of Chicago Medical Center.

It was hurtful because we remember our father as very healthy. He rode his bike to work, never smoked, never drank, said Brooke Casadaban.
Brooke and Leigh Casadaban say they're not satisfied with the answers they've been given so far.

How did their father, who had flu-like symptoms, enter the hospital a week ago Sunday morning and die 12 hours later with no one suspecting that it could be related to the plague?

Leigh, who is following in her father's footsteps, now attends her father's alma mater Massachusetts Institute of Technology, says it may be up to her to solve this.

I plan to go to the lab to read his papers and notes and go and really understand what he was trying to do, said Leigh Casadaban.

Leigh and Brooke say they've been told that their father is the first person in the Chicago area to die from this bacterium, an infection that is usually cured with antibiotics. They say they're not eager to file a lawsuit. But they are wondering now if someone at the hospital or the university should have been able to spot this before


TY

Demonstration video showing how to perform a serial dilution on a liquid food sample (in this case raw unpasteurised milk). The raw milk sample is diluted down to 1/1000 in a sterile diluent (Ringers solution). 1ml samples of each dilution are then used to prepare 2 sets of pour plates. A 0.1ml sample from the 1/10 and the 1/100 dilutions is used to generate spread plates.

For this method you require the following:
Sterile 1ml pipettes
A pipette pump
Sterile agar plates (for pour plates)
Poured agar plates (for spread plates)
A spreader (glass or metal)
A dish of lab ethanol
Bunsen burner
Glass Universal tubes with 9 ml of sterile diluent
Molten agar medium (20ml plate count agar per Universal tube - kept at 48C until ready to pour)

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Microbiology lab proceduree

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The opening video on a course on antibiotics, focusing on how morphology, gram stain, and other tests of bacteria can be used to create a clinically useful classification scheme.

UK scientists have created biodegradable, sustainable, microbeads which they say could reduce plastic pollution in our oceans. Jim Drury reports.

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A typical day in the life of a Microbiologist.

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This video discusses the cell wall structures of both Gram + and - bacteria